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src=\"https:\/\/shandong.nugene.com.cn\/wp-content\/uploads\/2024\/02\/image-10.png\" alt=\"\" class=\"wp-image-288\" srcset=\"https:\/\/shandong.nugene.com.cn\/wp-content\/uploads\/2024\/02\/image-10.png 554w, https:\/\/shandong.nugene.com.cn\/wp-content\/uploads\/2024\/02\/image-10-300x154.png 300w\" sizes=\"auto, (max-width: 554px) 100vw, 554px\" \/><\/figure>\n\n\n\n<p><strong>(2) \u53d1\u9175\u5de5\u827a\u54cd\u5e94\u9762\u4f18\u5316\u3002<\/strong><\/p>\n\n\n\n<p>\u5728\u5355\u56e0\u7d20\u8bd5\u9a8c\u7684\u57fa\u7840\u4e0a,\u5bf9\u82b1\u751f\u7ea2\u8863\u996e\u6599\u611f\u5b98\u54c1\u8d28\u6709\u5f71\u54cd\u7684\u4e94\u4e2a\u56e0\u7d20\u8fdb\u884c\u54cd\u5e94\u9762\u4f18\u5316,\u4f18\u5316\u53d1\u9175\u5de5\u827a\u3002<\/p>\n\n\n\n<p><strong>1.2.3 \u6d4b\u5b9a\u65b9\u6cd5<\/strong><\/p>\n\n\n\n<p>(1) \u82b1\u751f\u7ea2\u8863\u996e\u6599\u611f\u5b98\u8bc4\u4ef7\u3002 \u9009\u752810\u4f4d\u6d88\u8d39\u8005,\u6d88\u8d39\u8005\u8eab\u4f53\u5065\u5eb7,5\u75375\u5973,\u5e74\u9f84\u572820~40\u5c81,\u5206\u522b\u5bf9\u82b1\u751f\u7ea2\u8863\u53d1\u9175\u6db2\u7684\u53e3\u611f\u4f9d\u636e\u88682\u7684\u6807\u51c6\u8bc4\u5206,\u53d610\u4f4d\u8bc4\u4ef7\u8005\u7684\u5e73\u5747\u5f97\u5206\u4e3a\u6700\u7ec8\u5f97\u5206\u3002(2) \u539f\u82b1\u9752\u7d20\u7684\u6d4b\u5b9a\u65b9\u6cd5\u3002 \u91c7\u7528\u9999\u8349\u919b\u2014\u76d0\u9178\u6cd5\u6d4b\u5b9a\u63d0\u53d6\u6db2\u4e2d\u7684\u539f\u82b1\u9752\u7d20\u3002 \u53d6\u6837\u6db21.0 m L\u653e\u5165\u8bd5\u7ba1\u4e2d,\u52a0\u516540g\/L\u9999\u8349\u919b-\u7532\u9187\u6eb6\u6db23.0m L\u6df7\u5408,\u518d\u52a0\u51651.5 m L\u6d53\u76d0\u9178(36%~38%),\u7acb\u5373\u6df7\u5300\u3002 \u5ba4\u6e29\u4e0b\u663e\u827215 min\u5de6\u53f3,\u5728500nm\u6ce2\u957f\u4e0b\u6d4b\u5b9a\u5438\u5149\u503c,\u4ee540g\/L\u9999\u8349\u919b-\u7532\u9187\u6eb6\u6db2\u505a\u7a7a\u767d\u5bf9\u7167[17]\u3002<\/p>\n\n\n\n<p>(3)p H\u503c\u6d4b\u5b9a\u3002 \u91c7\u7528p H\u8ba1\u6d4b\u5b9a\u3002<\/p>\n\n\n\n<p>(4)HPLC\u8272\u8c31\u6761\u4ef6\u3002 \u8272\u8c31\u67f1\u4e3aAgilen C81\u67f1(4.6mm\u00d7150mm,5\u03bcm),\u6d41\u52a8\u76f80.1%\u78f7\u9178(A)\u548c\u4e59\u8148(B),\u68c0\u6d4b\u6ce2\u957f230nm,\u67f1\u6e2930 \u2103;\u8fdb\u6837\u91cf10\u03bcL;\u6d41\u901f0.8 m L\/min;\u68af\u5ea6\u6d17\u8131:0~7min,85% A,15% B;7~17min,70%~85% A,30%~15% B;17~27 min,51%~70% A,49%~30% B;27~30 min,45%~51% A,55% ~49% B;30~35 min,30%~45% A,70%~55%B;35~40min,30%~85%A,70%~15% B;40~45min,85% A,15% B\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"554\" height=\"196\" src=\"https:\/\/shandong.nugene.com.cn\/wp-content\/uploads\/2024\/02\/image-11.png\" alt=\"\" class=\"wp-image-289\" srcset=\"https:\/\/shandong.nugene.com.cn\/wp-content\/uploads\/2024\/02\/image-11.png 554w, https:\/\/shandong.nugene.com.cn\/wp-content\/uploads\/2024\/02\/image-11-300x106.png 300w\" sizes=\"auto, (max-width: 554px) 100vw, 554px\" \/><\/figure>\n\n\n\n<p><strong>1.3 \u6570\u636e\u5904\u7406\u65b9\u6cd5<\/strong><\/p>\n\n\n\n<p>\u5229\u7528Excel2010\u548cSPSS20.0\u8f6f\u4ef6\u8fdb\u884c\u6570\u636e\u5904\u7406\u548c\u7edf\u8ba1\u5206\u6790\u3002<\/p>\n\n\n\n<p><strong>2 \u7ed3\u679c\u4e0e\u5206\u6790<\/strong><\/p>\n\n\n\n<p><strong>2.1 \u6599\u6db2\u6bd4\u5bf9\u4e73\u9178\u83cc\u53d1\u9175\u82b1\u751f\u7ea2\u8863\u996e\u6599\u7684\u5f71\u54cd<\/strong><\/p>\n\n\n\n<p>\u7814\u7a76\u4e86\u4e0d\u540c\u6599\u6db2\u6bd4\u5bf9\u82b1\u751f\u7ea2\u8863\u4e73\u9178\u83cc\u53d1\u9175\u996e\u6599\u7684\u5f71\u54cd,\u7ed3\u679c\u89c1\u56fe1\u3002 \u4e73\u9178\u83cc\u53d1\u9175\u80fd\u591f\u660e\u663e\u6539\u5584\u82b1\u751f\u7ea2\u8863\u63d0\u53d6\u6db2\u7684\u611f\u5b98\u8bc4\u5206,\u4e0e\u5bf9\u7167\u76f8\u6bd4,\u4e0d\u540c\u6599\u6db2\u6bd4\u6761\u4ef6\u4e0b\u4e73\u9178\u83cc\u53d1\u9175\u82b1\u751f\u7ea2\u8863\u996e\u6599\u611f\u5b98\u5f97\u5206\u90fd\u660e\u663e\u9ad8\u4e8e\u5bf9\u7167,\u5728\u6599\u6db2\u6bd4\u4e3a1\u2236110\u65f6,\u611f\u5b98\u5f97\u5206\u6700\u9ad8(\u56fe1A)\u3002 \u53d1\u9175\u524d\u540e\u82b1\u751f\u7ea2\u8863\u6eb6\u6db2\u539f\u82b1\u9752\u7d20\u542b\u91cf\u672a\u89c1\u6709\u660e\u663e\u53d8\u5316(\u56fe1B),p H\u503c\u968f\u7740\u52a0\u6c34\u91cf\u589e\u52a0\u800c\u4e0b\u964d(\u56fe1C)\u3002 \u7531\u6b64\u5f97\u51fa,\u9002\u5b9c\u7684\u6599\u6db2\u6bd4\u4e3a1\u2236110\u3002<\/p>\n\n\n\n<p><strong>2.2 \u4e73\u9178\u83cc\u6dfb\u52a0\u91cf\u5bf9\u53d1\u9175\u82b1\u751f\u7ea2\u8863\u996e\u6599\u7684\u5f71\u54cd<\/strong><\/p>\n\n\n\n<p>\u56fe2A\u53ef\u770b\u51fa,\u6dfb\u52a0\u4e73\u9178\u83cc\u80fd\u663e\u8457\u63d0\u9ad8\u53d1\u9175\u6db2\u7684\u611f\u5b98\u5f97\u5206,\u8bf4\u660e\u4e73\u9178\u83cc\u53d1\u9175\u80fd\u6539\u5584\u82b1\u751f\u7ea2\u8863\u6eb6\u6db2\u7684\u6ecb\u5473\u54c1\u8d28\u3002 \u968f\u7740\u4e73\u9178\u83cc\u6dfb\u52a0\u91cf\u7684\u589e\u52a0,\u611f\u5b98\u8bc4\u5206\u5148\u7f13\u6162\u589e\u957f,\u5728\u6dfb\u52a0\u91cf0.7%~0.9%\u4e4b\u95f4\u589e\u957f\u663e\u8457,\u4e4b\u540e\u7565\u6709\u4e0b\u964d\u3002 \u53d1\u9175\u6db2\u4e2d\u539f\u82b1\u9752\u7d20\u6d53\u5ea6\u968f\u83cc\u5242\u6dfb\u52a0\u91cf\u7684\u589e\u52a0\u672a\u89c1\u6709\u663e\u8457\u53d8\u5316(\u56fe2B)\u3002<\/p>\n\n\n\n<p>\u7531\u56fe2C\u53ef\u770b\u51fa,p H\u503c\u968f\u7740\u83cc\u5242\u6dfb\u52a0\u91cf\u7684\u589e\u52a0\u5448\u663e\u8457\u4e0b\u964d\u8d8b\u52bf,\u57280.9%\u65f6\u8fbe\u5230\u6700\u4f4e,\u4e4b\u540e\u53d8\u5316\u4e0d\u663e\u8457,\u56e0\u6b64,\u8f83\u4f18\u7684\u83cc\u5242\u63a5\u79cd\u91cf\u4e3a0.9%\u3002<\/p>\n\n\n\n<p><strong>2.3 \u767d\u7cd6\u6dfb\u52a0\u91cf\u5bf9\u53d1\u9175\u82b1\u751f\u7ea2\u8863\u996e\u6599\u7684\u5f71\u54cd<\/strong><\/p>\n\n\n\n<p>\u56fe3A\u53ef\u770b\u51fa,\u968f\u7740\u767d\u7cd6\u6dfb\u52a0\u91cf\u7684\u589e\u52a0,\u611f\u5b98\u8bc4\u5206\u5148\u589e\u52a0\u540e\u964d\u4f4e,\u5f53\u767d\u7cd6\u6dfb\u52a0\u91cf\u8d85\u8fc710%\u65f6,\u7531\u4e8e\u83cc\u5242\u63a5\u79cd\u91cf\u548c\u53d1\u9175\u65f6\u95f4\u4e00\u5b9a,\u6b64\u65f6\u4e73\u9178\u751f\u6210\u91cf\u8d8b\u4e8e\u9971\u548c,\u4ea7\u54c1\u751c\u5ea6\u4e0a\u5347,\u5bfc\u81f4\u611f\u5b98\u8bc4\u5206\u4e0b\u964d\u3002\u53d1\u9175\u6db2\u4e2d\u539f\u82b1\u9752\u7d20\u6d53\u5ea6\u4e0e\u5bf9\u7167\u76f8\u6bd4\u672a\u89c1\u6709\u663e\u8457\u53d8\u5316(\u56fe3B)\u3002 \u56fe3C\u53ef\u770b\u51fa,p H\u503c\u968f\u767d\u7cd6\u6dfb\u52a0\u91cf\u589e\u52a0\u800c\u663e\u8457\u4e0b\u964d,\u5176\u539f\u56e0\u4e3a\u767d\u7cd6\u6dfb\u52a0\u91cf\u589e\u52a0\u6709\u5229\u4e8e\u4e73\u9178\u83cc\u7684\u53d1\u9175,\u800c\u4e73\u9178\u83cc\u53d1\u9175\u80fd\u4ea7\u751f\u5927\u91cf\u4e73\u9178,\u4ece\u800c\u4f7f\u53d1\u9175\u6db2\u7684p H\u503c\u4e0b\u964d\u3002 \u901a\u8fc7\u611f\u5b98\u3001p H\u503c\u7684\u7efc\u5408\u8bc4\u4ef7,\u9002\u5b9c\u7684\u767d\u7cd6\u6dfb\u52a0\u91cf\u4e3a10%\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"554\" height=\"341\" src=\"https:\/\/shandong.nugene.com.cn\/wp-content\/uploads\/2024\/02\/image-12.png\" alt=\"\" class=\"wp-image-290\" srcset=\"https:\/\/shandong.nugene.com.cn\/wp-content\/uploads\/2024\/02\/image-12.png 554w, https:\/\/shandong.nugene.com.cn\/wp-content\/uploads\/2024\/02\/image-12-300x185.png 300w\" sizes=\"auto, (max-width: 554px) 100vw, 554px\" \/><\/figure>\n\n\n\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"554\" height=\"324\" src=\"https:\/\/shandong.nugene.com.cn\/wp-content\/uploads\/2024\/02\/image-13.png\" alt=\"\" class=\"wp-image-291\" srcset=\"https:\/\/shandong.nugene.com.cn\/wp-content\/uploads\/2024\/02\/image-13.png 554w, https:\/\/shandong.nugene.com.cn\/wp-content\/uploads\/2024\/02\/image-13-300x175.png 300w\" sizes=\"auto, (max-width: 554px) 100vw, 554px\" \/><\/figure>\n\n\n\n<p><strong>2.4 \u53d1\u9175\u6e29\u5ea6\u5bf9\u4e73\u9178\u83cc\u53d1\u9175\u82b1\u751f\u7ea2\u8863\u996e\u6599\u7684\u5f71\u54cd<\/strong><\/p>\n\n\n\n<p>\u56fe4A\u53ef\u770b\u51fa,\u572836~44 \u2103\u8303\u56f4\u5185,\u82b1\u751f\u7ea2\u8863\u53d1\u9175\u6db2\u611f\u5b98\u8bc4\u5206\u968f\u53d1\u9175\u6e29\u5ea6\u7684\u53d8\u5316\u7565\u6709\u53d8\u5316,\u572842 \u2103\u65f6\u611f\u5b98\u5f97\u5206\u6700\u9ad8\u3002 \u53d1\u9175\u524d\u540e\u539f\u82b1\u9752\u7d20\u542b\u91cf\u6ca1\u6709\u663e\u8457\u53d8\u5316(\u56fe4B),\u540c\u65f6\u968f\u53d1\u9175\u6e29\u5ea6\u53d8\u5316,\u53d1\u9175\u6db2p H\u503c\u7684\u53d8\u5316\u4e5f\u5e76\u4e0d\u660e\u663e(\u56fe4B),\u56e0\u6b64,\u5728\u540e\u7eed\u7684\u54cd\u5e94\u9762\u4f18\u5316\u4e2d,\u56fa\u5b9a\u53d1\u9175\u6e29\u5ea6\u4e3a42\u2103\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"554\" height=\"362\" src=\"https:\/\/shandong.nugene.com.cn\/wp-content\/uploads\/2024\/02\/image-14.png\" alt=\"\" class=\"wp-image-292\" srcset=\"https:\/\/shandong.nugene.com.cn\/wp-content\/uploads\/2024\/02\/image-14.png 554w, https:\/\/shandong.nugene.com.cn\/wp-content\/uploads\/2024\/02\/image-14-300x196.png 300w\" sizes=\"auto, (max-width: 554px) 100vw, 554px\" \/><\/figure>\n\n\n\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"554\" height=\"329\" src=\"https:\/\/shandong.nugene.com.cn\/wp-content\/uploads\/2024\/02\/image-15.png\" alt=\"\" class=\"wp-image-293\" srcset=\"https:\/\/shandong.nugene.com.cn\/wp-content\/uploads\/2024\/02\/image-15.png 554w, https:\/\/shandong.nugene.com.cn\/wp-content\/uploads\/2024\/02\/image-15-300x178.png 300w\" sizes=\"auto, (max-width: 554px) 100vw, 554px\" \/><\/figure>\n\n\n\n<p><strong>2.5 \u53d1\u9175\u65f6\u95f4\u5bf9\u4e73\u9178\u83cc\u53d1\u9175\u82b1\u751f\u7ea2\u8863\u996e\u6599\u7684\u5f71\u54cd<\/strong><\/p>\n\n\n\n<p>\u56fe5A\u53ef\u89c1,\u968f\u7740\u53d1\u9175\u65f6\u95f4\u589e\u52a0,\u611f\u5b98\u5f97\u5206\u5148\u589e\u52a0\u540e\u4e0b\u964d,\u5728\u53d1\u9175\u65f6\u95f4\u4e3a8h\u65f6\u8fbe\u5230\u6700\u9ad8,\u4e4b\u540e\u663e\u8457\u4e0b\u964d\u3002 \u53d1\u9175\u6db2\u4e2d\u539f\u82b1\u9752\u7d20\u6d53\u5ea6\u968f\u53d1\u9175\u65f6\u95f4\u7684\u53d8\u5316\u5e76\u672a\u6709\u663e\u8457\u53d8\u5316(\u56fe5B)\u3002 \u56fe5C\u53ef\u89c1,p H\u503c\u968f\u53d1\u9175\u65f6\u95f4\u7684\u589e\u52a0\u5448\u663e\u8457\u4e0b\u964d\u8d8b\u52bf,\u539f\u56e0\u662f\u968f\u53d1\u9175\u65f6\u95f4\u589e\u52a0,\u53d1\u9175\u4ea7\u7269\u4e2d\u4e73\u9178\u542b\u91cf\u4e5f\u968f\u4e4b\u589e\u52a0,\u6545p H\u503c\u663e\u8457\u4e0b\u964d\u3002 \u7efc\u4e0a\u6240\u8ff0,\u9002\u5b9c\u53d1\u9175\u65f6\u95f4\u4e3a8h\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"554\" height=\"330\" src=\"https:\/\/shandong.nugene.com.cn\/wp-content\/uploads\/2024\/02\/image-16.png\" alt=\"\" class=\"wp-image-294\" srcset=\"https:\/\/shandong.nugene.com.cn\/wp-content\/uploads\/2024\/02\/image-16.png 554w, https:\/\/shandong.nugene.com.cn\/wp-content\/uploads\/2024\/02\/image-16-300x179.png 300w\" sizes=\"auto, (max-width: 554px) 100vw, 554px\" \/><\/figure>\n\n\n\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"554\" height=\"438\" src=\"https:\/\/shandong.nugene.com.cn\/wp-content\/uploads\/2024\/02\/image-17.png\" alt=\"\" class=\"wp-image-295\" srcset=\"https:\/\/shandong.nugene.com.cn\/wp-content\/uploads\/2024\/02\/image-17.png 554w, https:\/\/shandong.nugene.com.cn\/wp-content\/uploads\/2024\/02\/image-17-300x237.png 300w\" sizes=\"auto, (max-width: 554px) 100vw, 554px\" \/><\/figure>\n\n\n\n<p><strong>\u00a02.6 \u54cd\u5e94\u9762\u4f18\u5316<\/strong><\/p>\n\n\n\n<p>\u91c7\u7528Box-Behnken\u4e2d\u5fc3\u7ec4\u5408\u8bd5\u9a8c\u8bbe\u8ba1\u539f\u7406,\u7ed3\u5408\u5355\u56e0\u7d20\u8bd5\u9a8c\u7ed3\u679c,\u56fa\u5b9a\u53d1\u9175\u6e29\u5ea6\u4e3a42 \u2103,\u8003\u5bdf\u6599\u6db2\u6bd4(A)\u3001\u4e73\u9178\u83cc\u7528\u91cf(B)\u3001\u767d\u7cd6\u7528\u91cf(C)\u548c\u53d1\u9175\u65f6\u95f4(D)\u5bf9\u53d1\u9175\u82b1\u751f\u7ea2\u8863\u611f\u5b98(Y)\u7684\u5f71\u54cd,\u8bd5\u9a8c\u8bbe\u8ba1\u4e0e\u7ed3\u679c\u89c1\u88683\u3002<\/p>\n\n\n\n<p><strong>2.6.1 \u6a21\u578b\u7684\u5efa\u7acb\u53ca\u5176\u663e\u8457\u6027\u68c0\u9a8c<\/strong><\/p>\n\n\n\n<p>\u6839\u636e\u8bd5\u9a8c\u6570\u636e,\u5229\u7528Design-Expert8.0\u8f6f\u4ef6\u8fdb\u884c\u4e8c\u6b21\u591a\u9879\u5f0f\u56de\u5f52\u62df\u5408,\u56de\u5f52\u65b9\u7a0b\u4e3a:Y= 88.8+2.17A-1.75B+0.76C-0.083D-0.5AB+1.5AC+1.0BC+1.75BD+2.0CD+4.97A2+1.1B2-0.025C2-0.9D2<\/p>\n\n\n\n<p>\u901a\u8fc7Design-Expert\u8f6f\u4ef6\u8fdb\u884c\u65b9\u5dee\u5206\u6790\u6765\u9a8c\u8bc1\u56de\u5f52\u6a21\u578b\u53ca\u5404\u53c2\u6570\u7684\u663e\u8457\u5ea6,\u7ed3\u679c\u89c1\u88684\u3002<\/p>\n\n\n\n<p>\u7531\u65b9\u5dee\u5206\u6790\u53ef\u770b\u51fa,\u6a21\u578bP \u503c\u5c0f\u4e8e0.01,\u8868\u660e\u8be5\u6a21\u578b\u65b9\u7a0b\u6781\u663e\u8457,\u4e0d\u540c\u5904\u7406\u95f4\u7684\u5dee\u5f02\u9ad8\u5ea6\u663e\u8457;\u6a21\u578b\u5931\u62df\u9879\u7684P \u503c\u4e3a0.2693>0.05,\u6a21\u578b\u5931\u62df\u9879\u4e0d\u663e\u8457,\u8bf4\u660e\u6a21\u578b\u9009\u62e9\u5408\u9002,\u4e14\u6a21\u578bR2 95.21%,\u5927\u4e8e90%,\u8bf4\u660e\u6a21\u578b\u62df\u5408\u7a0b\u5ea6\u826f\u597d,\u8bd5\u9a8c\u8bef\u5dee\u5c0f,\u8be5\u6a21\u578b\u662f\u5408\u9002\u7684;\u53d8\u5f02\u7cfb\u6570\u53cd\u6620\u6a21\u578b\u7684\u7f6e\u4fe1\u5ea6,\u53d8\u5f02\u7cfb\u6570\u8d8a\u4f4e,\u6a21\u578b\u7684\u7f6e\u4fe1\u5ea6\u8d8a\u597d,\u672c\u8bd5\u9a8c\u53d8\u5f02\u7cfb\u6570\u4e3a1.2%,\u8bf4\u660e\u6a21\u578b\u65b9\u7a0b\u80fd\u5f88\u597d\u53cd\u5e94\u771f\u5b9e\u7684\u8bd5\u9a8c\u503c\u3002\u56e0\u6b64\u53ef\u7528\u8be5\u6a21\u578b\u65b9\u7a0b\u6765\u5206\u6790\u548c\u9884\u6d4b\u4e73\u9178\u83cc\u53d1\u9175\u82b1\u751f\u7ea2\u8863\u996e\u6599\u7684\u611f\u5b98\u54c1\u8d28\u53d8\u5316\u3002 \u7531\u88684\u7684P \u503c\u53ef\u77e5\u9053,\u65b9\u7a0b\u4e2d A\u3001B\u3001BD\u3001CD \u5bf9Y \u503c\u7684\u5f71\u54cd\u6781\u663e\u8457,C \u548cAC \u5bf9Y \u503c\u7684\u5f71\u54cd\u663e\u8457,\u8868\u660e\u8bd5\u9a8c\u56e0\u7d20\u5bf9\u54cd\u5e94\u503c\u7684\u5f71\u54cd\u4e0d\u662f\u7b80\u5355\u7684\u7ebf\u6027\u5173\u7cfb,\u4ea4\u4e92\u9879\u548c\u4e8c\u6b21\u9879\u4e5f\u6709\u663e\u8457\u5f71\u54cd\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"554\" height=\"413\" src=\"https:\/\/shandong.nugene.com.cn\/wp-content\/uploads\/2024\/02\/image-18.png\" alt=\"\" class=\"wp-image-296\" srcset=\"https:\/\/shandong.nugene.com.cn\/wp-content\/uploads\/2024\/02\/image-18.png 554w, https:\/\/shandong.nugene.com.cn\/wp-content\/uploads\/2024\/02\/image-18-300x224.png 300w\" sizes=\"auto, (max-width: 554px) 100vw, 554px\" \/><\/figure>\n\n\n\n<p><strong>2.6.2 \u54cd\u5e94\u9762\u4f18\u5316\u7ed3\u679c\u5206\u6790<\/strong><\/p>\n\n\n\n<p>\u6839\u636e\u56de\u5f52\u5206\u6790\u7ed3\u679c\u505a\u6a21\u578b\u54cd\u5e94\u9762\u548c\u7b49\u9ad8\u7ebf\u56fe(\u56fe6)\u3002 \u7531\u56fe6A\u53ef\u89c1,\u4e73\u9178\u83cc\u7684\u6dfb\u52a0\u91cf\u5904\u4e8e\u8f83\u4f4e\u6c34\u5e73\u65f6,\u53d1\u9175\u6db2\u611f\u5b98\u8bc4\u5206\u968f\u6599\u6db2\u6bd4\u7684\u589e\u52a0\u800c\u589e\u52a0,\u4f46\u5f53\u4e73\u9178\u83cc\u7684\u6dfb\u52a0\u91cf\u5904\u4e8e\u9ad8\u6c34\u5e73\u65f6,\u53d1\u9175\u6db2\u611f\u5b98\u8bc4\u5206\u968f\u6599\u6db2 \u6bd4 \u7684 \u589e \u52a0 \u800c \u5148 \u964d \u4f4e \u540e \u589e \u52a0;\u7531 \u56fe6B\u53ef\u89c1,\u4e73\u9178\u83cc\u7684\u6dfb\u52a0\u91cf\u5904\u4e8e\u4e2d\u95f4\u6c34\u5e73,\u968f\u53d1\u9175\u65f6\u95f4\u7684\u589e\u52a0,\u611f\u5b98\u8bc4\u5206\u5448\u73b0\u5148\u589e\u52a0\u540e\u51cf\u5c11;\u4f46\u5f53\u4e73\u9178\u83cc\u7684\u6dfb\u52a0\u91cf\u5904\u4e8e\u9ad8\u6c34\u5e73\u65f6,\u968f\u53d1\u9175\u65f6\u95f4\u7684\u589e\u52a0\u611f\u5b98\u8bc4\u5206\u9010\u6b65\u589e\u52a0;\u800c\u56fe6C\u8868\u660e,\u767d\u7cd6\u6dfb\u52a0\u91cf\u5904\u4e8e\u4f4e\u54cd\u5e94\u9762\u6a2a\u5750\u6807\u4e2d\u7684-1~1\u4ee3\u8868\u5f52\u4e00\u5316\u53d8\u91cf\u6c34\u5e73\u3002<\/p>\n\n\n\n<p>Note:-1~1ofabscissaerepresentthelevelofnormalizedvariablesoftestfactors\u6c34\u5e73\u65f6,\u611f\u5b98\u8bc4\u5206\u968f\u53d1\u9175\u65f6 \u95f4\u7684\u589e\u52a0\u800c\u589e\u52a0,\u5f53\u767d\u7cd6\u6dfb\u52a0 \u91cf \u5904 \u4e8e \u9ad8 \u6c34 \u5e73 \u65f6,\u611f \u5b98 \u8bc4 \u5206 \u968f \u53d1 \u9175 \u65f6\u95f4\u7684\u589e\u52a0\u800c\u964d\u4f4e\u3002\u8fdb\u4e00\u6b65\u7528Design-Expert\u8f6f\u4ef6\u5bf9\u8bd5\u9a8c\u6a21\u578b\u8fdb\u884c\u5178\u578b\u6027\u5206\u6790,\u4ee5\u83b7\u5f97\u6700\u4f18\u7684\u63d0\u53d6\u6761\u4ef6\u3002<\/p>\n\n\n\n<p>\u7ecf\u5206\u6790,\u5728 A=1.0\u3001B=-0.57\u3001C= 0.57\u3001D=0.19,\u5373\u5728\u6599\u6db2\u6bd4\u4e3a1\u2236130\u3001\u4e73\u9178\u83cc\u6dfb\u52a0\u91cf\u4e3a0.786%\u3001\u767d\u7cd6\u6dfb\u52a0\u91cf\u4e3a11.14%,\u53d1\u9175\u65f6\u95f4\u4e3a8.38h\u65f6\u5f97\u5230\u7406\u8bba\u6700\u5927\u503c(\u611f\u5b98\u8bc4\u5206)\u4e3a98.4\u3002<\/p>\n\n\n\n<p><strong>2.6.3 \u6a21\u578b\u9a8c\u8bc1\u4e3a\u9a8c\u8bc1<\/strong><\/p>\n\n\n\n<p>\u54cd\u5e94\u9762\u6a21\u578b\u7684\u53ef\u884c\u6027,\u91c7\u7528\u5f97\u5230\u7684\u6700\u4f73\u53d1\u9175\u6761\u4ef6\u8fdb\u884c\u4e73\u9178\u83cc\u53d1\u9175\u82b1\u751f\u7ea2\u8863\u6eb6\u6db2,\u540c\u65f6\u8003\u8651\u5230\u5b9e\u9645\u64cd\u4f5c\u548c\u751f\u4ea7\u7684\u4fbf\u5229\u6027,\u5c06\u53d1\u9175\u6761\u4ef6\u4fee\u6b63\u4e3a\u6599\u6db2\u6bd4\u4e3a1\u2236130\u3001\u4e73\u9178\u83cc\u6dfb\u52a0\u91cf\u4e3a0.79%\u3001\u767d\u7cd6\u6dfb\u52a0\u91cf\u4e3a11.0%,\u53d1\u9175\u65f6\u95f48.4h\u3002<\/p>\n\n\n\n<p>\u4e09\u6b21\u5e73\u884c\u8bd5\u9a8c\u5f97\u5230\u7684\u5b9e\u9645\u5e73\u5747\u503c(\u611f \u5b98\u8bc4\u5206)\u4e3a97.00\u00b10.76,\u4e0e\u7406\u8bba\u503c\u76f8\u5dee1.42%,\u53d1\u9175\u6db2p H\u503c\u4e3a6.32\u00b10.04,\u8868\u660e\u54cd\u5e94\u9762\u6cd5\u5bf9\u4e73\u9178\u83cc\u53d1\u9175\u82b1\u751f\u7ea2\u8863\u7684\u4f18\u5316\u662f\u53ef\u884c\u7684,\u5f97\u5230\u7684\u53d1\u9175\u5de5\u827a\u6761\u4ef6\u5177\u6709\u5b9e\u9645\u5e94\u7528\u4ef7\u503c\u3002<\/p>\n\n\n\n<p><strong>2.7 \u4e73\u9178\u83cc\u53d1\u9175\u5bf9\u82b1\u751f\u7ea2\u8863\u539f\u82b1\u9752\u7d20\u6210\u5206\u53ca\u7ed3\u6784\u5f71\u54cd\u7684\u521d\u6b65\u7814\u7a76<\/strong><\/p>\n\n\n\n<p>\u82b1\u751f\u7ea2\u8863\u63d0\u53d6\u7269\u4e3b\u8981\u6210\u5206\u662f\u539f\u82b1\u9752\u7d20,\u4e14\u4e73\u9178\u83cc\u53d1\u9175\u80fd\u663e\u8457\u6539\u5584\u82b1\u751f\u7ea2\u8863\u63d0\u53d6\u7269\u7684\u6ecb\u5473\u54c1\u8d28,\u5229\u7528HPLC\u6d4b\u5b9a\u53d1\u9175\u8fc7\u7a0b\u5bf9\u82b1\u751f\u7ea2\u8863\u539f\u82b1\u9752\u7d20\u7684\u5f71\u54cd,\u540c\u65f6\u4ee5\u82b1\u751f\u7ea2\u8863\u539f\u82b1\u9752\u7d20\u548c\u4e73\u9178\u83cc\u6eb6\u6db2\u505a\u5bf9\u7167,\u521d\u6b65\u63a2\u7a76\u4e73\u9178\u83cc\u53d1\u9175\u6539\u5584\u82b1\u751f\u7ea2\u8863\u63d0\u53d6\u7269\u6ecb\u5473\u54c1\u8d28\u7684\u673a\u7406\u3002 \u7531\u56fe7\u53ef\u770b\u51fa,\u4e73\u9178\u83cc\u53d1\u9175\u82b1\u751f\u7ea2\u8863\u539f\u82b1\u9752\u7d20\u7684HPLC\u56fe\u548c\u82b1\u751f\u7ea2\u8863\u539f\u82b1\u9752\u7d20\u7684HPLC\u56fe\u57fa\u672c\u4e00\u81f4,\u8bf4\u660e\u4e73\u9178\u83cc\u53d1\u9175\u5e76\u672a\u5bf9\u539f\u82b1\u9752\u7d20\u542b\u91cf\u548c\u7ed3\u6784\u4ea7\u751f\u663e\u8457\u5f71\u54cd\u3002 \u82b1\u751f\u7ea2\u8863\u539f\u82b1\u9752\u7d20\u5177\u6709\u5f88\u5f3a\u6da9\u5473,\u4e73\u9178\u83cc\u53d1\u9175\u80fd\u663e\u8457\u6539\u5584\u5176\u53e3\u611f,\u4f46\u5176\u7ed3\u6784\u6216\u8005\u542b\u91cf\u5e76\u672a\u53d1\u751f\u6539\u53d8,\u5176\u5177\u4f53\u539f\u56e0\u9700\u8981\u8fdb\u4e00\u6b65\u7814\u7a76\u548c\u63a2\u8ba8\u3002<\/p>\n\n\n\n<p><strong>3 \u8ba8\u8bba\u4e0e\u7ed3\u8bba<\/strong><\/p>\n\n\n\n<p>\u4e73\u9178\u83cc\u53d1\u9175\u82b1\u751f\u7ea2\u8863\u539f\u82b1\u9752\u7d20\u7684\u8fc7\u7a0b\u4e2d,\u4e73\u9178\u83cc\u4e0e\u539f\u82b1\u9752\u7d20\u76f8\u4e92\u4f5c\u7528,\u76f8\u4e92\u5f71\u54cd\u3002 \u4e73\u9178\u83cc\u53d1\u9175\u80fd\u660e\u663e\u6539\u5584\u82b1\u751f\u7ea2 \u8863\u539f\u82b1\u9752\u7d20\u6eb6\u6db2\u7684\u6ecb\u5473\u54c1\u8d28;\u539f\u82b1\u9752\u7d20\u5177\u6709\u4e00\u5b9a\u7684\u6291\u83cc\u4f5c\u7528,\u4f4e\u6d53\u5ea6\u7684\u539f\u82b1\u9752\u7d20\u6709\u5229\u4e8e\u4e73\u9178\u83cc\u7684\u751f\u957f,\u8f83\u9ad8\u6d53\u5ea6\u7684\u539f\u82b1\u9752\u7d20\u5bf9\u4e73\u9178\u83cc\u6709\u4e00\u5b9a\u7684\u6291\u5236\u4f5c\u7528[19-20]\u3002 \u672c \u8bd5\u9a8c\u4e2d\u968f\u7740\u52a0\u6c34\u91cf\u589e\u52a0,\u53d1\u9175\u6db2\u7684p 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